報告題目：Carbon dots doped with nitrogen as an ultrasensitive fluorescent probe for thrombin activity monitoring and inhibitor screening

報  告 人：趙艷

報告時間：2023年5月17日下午14:00

報告地點：化學實驗中心417

主辦單位：理學院

報告人簡介：

趙艷，女，博士，教授，碩士生導師。主要研究方向為新型納米材料的制備和性質研究及其在生物活性成分、污染物分析方面的應用。先后在Food Chem.、Talanta、Microchem. J.等國際期刊發表SCI論文10余篇。

報告摘要：

A simple and sensitive fluorometric assay based on nitrogen-doped carbon dots (N-CDs) was developed for the determination of thrombin (TB) activity in human serum samples and living cells. The novel N-CDs were prepared by a facile one-pot hydrothermal method using 1,2-ethylenediamine and levodopa as precursors. Such N-CDs exhibited green fluorescence with excitation/emission peaks at 390/520 nm and a high fluorescence quantum yield of approximately 39.2%. H-D-Phenylalanyl-L-pipecolyl-Larginine-p-nitroaniline-dihydrochloride (S-2238) was hydrolyzed by TB to produce p-nitroaniline which was capable of quenching the fluorescence of N-CDs due to an inner filter effect. This assay was used to detect TB activity with a low detection limit of 11.3 fM. The proposed sensing method was then expanded to the TB inhibitor screening and exhibited excellent applicability. As a typical TB inhibitor, argatroban was determined in a concentration as low as 1.43 nM. The method has also been successfully employed for the determination of TB activity in living Hela cells. This work showed significant potential for TB activity assay in clinical and biomedicine applications.